What is Humanin?
Humanin (Humanina) is a mitochondrial-derived peptide (MDP) studied as a cytoprotective survival peptide. It is researched for metabolic and neuroprotective effects and complements MOTS-c and Epithalon in a mitochondrial longevity stack. The potent S14G analog (HNG) is the form usually sold.
Reconstitution
Humanin reconstitutes with bacteriostatic water. Draw 2mL down the inside wall of the vial, swirl gently until fully dissolved, and refrigerate. Do not shake.
Quick Reference
| Parameter | Value |
|---|---|
| Peptide Amount | 10mg |
| Reconstitution Solvent | Bacteriostatic water |
| Recommended Volume | 2mL |
| Resulting Concentration | 5mg/mL |
| Typical Research Dose | varies |
| Frequency | daily |
Reconstitution Guide
Materials Needed
- Humanin 10mg lyophilized vial
- Bacteriostatic water (BAC water)
- Insulin syringes (U-100, 29-31 gauge)
- Alcohol swabs
- Sharps container
Step-by-Step Instructions
- Prepare workspace — Clean environment, gather materials, thorough handwashing.
- Clean vial stoppers — Wipe both vials with alcohol swabs. Allow complete drying.
- Draw BAC water — Draw 2mL bacteriostatic water using a sterile syringe.
- Add to peptide — Inject slowly down the inside wall of the vial. Do not spray directly onto the powder.
- Dissolve — Allow a few minutes for complete dissolution. Gentle swirl only — no shaking.
- Verify — Solution should be clear.
- Label and store — Note reconstitution date. Refrigerate immediately.
Dosage Protocol
Concentration Calculations
With 10mg peptide in 2mL BAC water:
- Concentration: 5mg/mL (5000mcg/mL)
- Per unit (U-100 syringe): 50mcg per unit
Run your exact vial and water combination through the peptide reconstitution calculator to rebuild this table for your setup.
Common Dose Calculations
| Desired Dose | Draw Volume | Syringe Units |
|---|---|---|
| 1mg | 0.20mL | 20 units |
| 2mg | 0.40mL | 40 units |
Research Considerations
Mechanism
Humanin is encoded within the mitochondrial 16S rRNA region and acts on cell-survival pathways, buffering cells against apoptotic and metabolic stress in preclinical models.
Sourcing for Research
Source from verified suppliers that provide third-party testing and certificates of analysis (COA) for each batch. Compare purity documentation, shipping conditions, and batch traceability before purchasing research materials.
Storage Requirements
Lyophilized (Powder)
- Temperature: -20°C or colder
- Duration: 2+ years
- Conditions: Dry, dark, protected from moisture
Reconstituted
- Temperature: 2-8°C (refrigerated)
- Duration: 2-3 weeks
- Conditions: Protected from light, never freeze
Safety Considerations
- Humanin is a research compound
- Not approved for any indication in any jurisdiction
- Research use only — not for human consumption
- Use sterile technique; never reuse needles or syringes
Scientific References
- Research literature on Humanin and its proposed mechanism of action.
- Peer-reviewed and preclinical sources on the compound class discussed above.
Last updated: 2026-07-20.
Disclaimer: This information is for educational and research purposes only. Humanin is a research compound and is not approved for any use. Research-grade peptides are not intended for human consumption. Always follow applicable laws and institutional guidelines.