TB-500 2mg Dosage & Reconstitution Guide

What is TB-500?

TB-500 is a synthetic fragment of Thymosin Beta-4 (Tβ4), a naturally occurring 43-amino acid peptide found in virtually all human tissues. TB-500 specifically corresponds to the active region of Tβ4 responsible for actin binding and cell migration.

The peptide plays a critical role in tissue repair by promoting cell migration, blood vessel formation, and reducing inflammation. Its mechanism centers on sequestering actin monomers — a process essential for cell motility and wound healing.

TB-500 has systemic effects due to its low molecular weight, allowing it to travel through tissues and exert healing effects at sites distant from the injection point.

Quick Reference

Reconstitution Guide

Materials Needed

• TB-500 2mg lyophilized vial
• Bacteriostatic water (BAC water)
• Insulin syringes (U-100, 29-31 gauge)
• Alcohol swabs
• Sharps container

Step-by-Step Instructions

1. Prepare your workspace — Clean your work area and gather all materials. Wash hands thoroughly.

2. Clean the vial stoppers — Wipe the TB-500 vial stopper and BAC water vial with alcohol swabs. Allow to dry completely.

3. Draw the BAC water — Using a sterile syringe, draw 1mL of bacteriostatic water.

4. Add water to peptide vial — Insert the needle into the TB-500 vial and slowly inject the BAC water down the inside wall of the vial. Do not spray directly onto the powder.

5. Allow to dissolve — TB-500 may take 5-10 minutes to fully dissolve. Gently swirl occasionally — never shake vigorously.

6. Verify dissolution — The solution should be completely clear and colorless. If cloudy or particles remain, continue gentle swirling.

7. Label and store — Write the reconstitution date on the vial. Store in refrigerator immediately.

Dosage Protocol

Concentration Calculations

With 2mg peptide in 1mL BAC water:

• Resulting Concentration: 2.00mg/mL = 2000mcg/mL
• Per unit (U-100 syringe): 20.0mcg per unit

Common Dose Calculations

Injection Guide

Subcutaneous Administration

1. Clean the injection site with an alcohol swab
2. Pinch a fold of skin (abdomen, thigh, or upper arm)
3. Insert needle at 90° angle
4. Inject slowly and steadily
5. Wait 5-10 seconds before withdrawing needle
6. Apply light pressure if needed — do not rub

Site Rotation

Rotate between these areas:

• Abdomen (2+ inches from navel) — most common
• Outer thigh (front/outer area)
• Back of upper arm

Storage Requirements

Lyophilized (Powder Form)

Reconstituted (Mixed with BAC Water)

Research Considerations

Mechanism of Action

Actin sequestration and cell migration promotion, angiogenesis, anti-inflammatory signaling, stem cell differentiation support

Key Research Findings

Research demonstrated accelerated wound healing, reduced scar formation, and improved cardiac function after injury in preclinical models.

Safety & Handling

• Use sterile technique for all preparations
• Never reuse needles or syringes
• Dispose of sharps properly in designated containers
• Store away from children and unauthorized persons
• TB-500 is for research purposes only

Sourcing for Research

Source from verified suppliers that provide third-party testing and certificates of analysis (COA) for each batch. Compare purity documentation, shipping conditions, and batch traceability before purchasing research materials.

Scientific References

1. Thymosin β4: Roles in Development, Repair, and Engineering of the Cardiovascular System — Ann NY Acad Sci

Last updated: February 2026

This information is for educational and research purposes only. TB-500 is a research peptide not intended for human consumption. Always follow applicable laws and institutional guidelines.