Glutathione 1500mg Dosage & Mixing Guide

What is Glutathione?

Glutathione (GSH, glutation, Glutathion) is the endogenous tripeptide gamma-Glu-Cys-Gly, the cell’s main thiol antioxidant and the reducing partner in the GSH/GSSG redox couple. It is a molecule the body synthesises and recycles continuously, which is why the injectable form is sold by the gram rather than by the milligram.

The 1500mg vial is the large presentation, aimed at multi-dose protocols or at the higher single doses used in the human intravenous literature. It sits alongside NAD+ as the other gram-scale entry in this catalogue, and the smaller 600mg vial covers the same ground at a third of the mass.

Read this before touching a syringe. A 1500mg vial carries roughly 250 times the mass of a typical research peptide vial. The “mcg per insulin unit” framing used elsewhere on this site is the wrong instrument here — at any realistic reconstitution volume a single insulin unit holds well over a milligram, and one research dose would empty several full U-100 syringes. Everything below is in mg/mL and mL. Use a 5mL or 10mL Luer-lock syringe.

Reconstitution

Glutathione reconstitutes with bacteriostatic water. Draw 10mL down the inside wall of the vial in three or four portions, swirling gently between additions until fully dissolved, then refrigerate. Do not shake. At this mass the powder bed is substantial and dissolution is slower than for a small peptide vial — give it time rather than agitation.

Confirm the vial’s nominal capacity first. A 1500mg lyophilised charge is usually filled into a 10mL or larger vial, but the powder itself occupies real volume, so add water incrementally and stop if you run out of headspace.

Once in solution, glutathione is a thiol in water and oxidises to GSSG over time. Cold, dark, and low air contact are the three things that matter.

Quick Reference

Reconstitution Guide

Materials Needed

• Glutathione 1500mg lyophilized vial
• Bacteriostatic water (BAC water) — a 30mL vial is the practical choice at this volume
• 5mL and 10mL Luer-lock syringes with drawing needles
• Alcohol swabs
• Sharps container

Step-by-Step Instructions

1. Prepare workspace — Clean environment, gather materials, thorough handwashing.
2. Clean vial stoppers — Wipe both vials with alcohol swabs. Allow complete drying.
3. Check capacity — Confirm the vial can take 10mL on top of the powder bed. Add in stages and reassess.
4. Draw BAC water — Draw 10mL bacteriostatic water, in three or four syringe loads.
5. Add to peptide — Inject slowly down the inside wall. Do not spray directly onto the powder.
6. Dissolve — Swirl gently between additions; allow five to ten minutes overall. No shaking.
7. Verify — Solution should be clear and colourless to very faintly straw-coloured. Discard if cloudy or if particles persist.
8. Label and store — Note reconstitution date. Refrigerate immediately, protected from light.

Dosage Protocol

Concentration Calculations

With 1500mg peptide in 10mL BAC water:

• Concentration: 1500mg / 10mL = 150mg/mL (150,000mcg/mL)
• Per unit (U-100 syringe): 150mg/mL x 1000 / 100 = 1500mcg per unit — 1.5mg in a single tick mark

Which is exactly why insulin units are the wrong tool for this vial. A 600mg dose is 400 units, or four complete 1mL insulin syringes back to back; a full-vial 1500mg dose is a thousand units. The same draw expressed in millilitres is 4.00mL and 10.00mL — one reading on a 5mL or 10mL syringe, no arithmetic, no chain of refills. Read mL.

If you want the same 100mg/mL concentration as the 600mg vial so both sizes share one dose table, use 15mL of BAC water instead. Draw volumes then become dose / 100.

Run your exact vial and water combination through the peptide reconstitution calculator to rebuild this table for your setup.

Common Dose Calculations

At 150mg/mL:

At 300mg per administration the vial yields 5 doses; at 500mg it yields 3.

Research Considerations

Mechanism

The reactive centre of glutathione is the cysteine thiol. GSH is the reducing cofactor for the glutathione peroxidases, which detoxify hydrogen peroxide and lipid hydroperoxides, and the conjugating substrate for the glutathione S-transferases in phase II metabolism. Glutathione reductase regenerates GSH from GSSG using NADPH, which is why glutathione and NAD(P)-pool research tend to be read together.

The caveat that matters at gram doses: exogenous glutathione is extensively cleaved outside the cell by gamma-glutamyl transpeptidase, and cells then rebuild their own GSH from the released amino acids. In the human pharmacokinetic study by Aebi and colleagues, a 2g/m² intravenous dose raised plasma cysteine more than tenfold and increased urinary glutathione excretion roughly 300-fold within 90 minutes, against a measured plasma half-life of about 14 minutes. Injected glutathione is best understood as a fast, largely renally cleared cysteine delivery vehicle rather than as a direct top-up of the intracellular pool.

Where the human evidence actually stands:

• Pharmacokinetics — established in humans. 14.1 +/- 9.2 min plasma half-life, volume of distribution 176 +/- 107 mL/kg, n=10.
• Neurology — a randomised, double-blind pilot dosed 1400mg IV three times weekly for four weeks in Parkinson’s disease. Well tolerated, no significant efficacy separation from placebo. An intranasal Phase IIb reached the same conclusion.
• Dermatology / melanin index — the controlled evidence is mostly for oral dosing at 250-500mg daily. The intravenous skin-lightening literature is thin and the systematic reviews are openly critical of its design quality.
• Metabolic, hepatic and performance claims — preclinical or mechanistic. Hypothesis-generating, not settled.

Anyone comparing gram-scale vials should note that the doses used in the published human work (1400mg IV, or 2g/m²) are in the same range as this vial’s total contents — one vial is roughly one literature dose, not a month of them.

Sourcing for Research

Source from verified suppliers that provide third-party testing and certificates of analysis (COA) for each batch. For glutathione, ask specifically what proportion of the assayed mass is reduced GSH versus oxidised GSSG — at 1500mg per vial, a few percent of oxidation is tens of milligrams. Compare purity documentation, shipping conditions, and batch traceability before purchasing research materials.

Storage Requirements

Lyophilized (Powder)

• Temperature: -20°C or colder
• Duration: 2+ years
• Conditions: Dry, dark, protected from moisture

Reconstituted

• Temperature: 2-8°C (refrigerated)
• Duration: 2-4 weeks
• Conditions: Protected from light, never freeze, minimise headspace air

Safety Considerations

• Glutathione is a research compound
• Not approved for any indication in any jurisdiction
• Research use only — not for human consumption
• Use sterile technique; never reuse needles or syringes

Scientific References

1. Aebi S, Assereto R, Lauterburg BH. High-dose intravenous glutathione in man. Pharmacokinetics and effects on cyst(e)ine in plasma and urine. Eur J Clin Invest. 1991;21(1):103-110.
2. Hauser RA, Lyons KE, McClain T, Carter S, Perlmutter D. Randomized, double-blind, pilot evaluation of intravenous glutathione in Parkinson’s disease. Mov Disord. 2009;24(7):979-983.
3. Mischley LK, Lau RC, Shankland EG, Wilbur TK, Padowski JM. Phase IIb study of intranasal glutathione in Parkinson’s disease. J Parkinsons Dis. 2017;7(2):289-299.
4. Sarkar R, et al. Glutathione as a skin-lightening agent and in melasma: a systematic review. Int J Dermatol. 2025.
5. Wendel A, Cikryt P. The level and half-life of glutathione in human plasma. FEBS Lett. 1980;120(2):209-211.

Last updated: 2026-07-21.

Disclaimer: This information is for educational and research purposes only. Glutathione is a research compound and is not approved for any use. Research-grade peptides are not intended for human consumption. Always follow applicable laws and institutional guidelines.