What is FOXO4-DRI?
FOXO4-DRI is a D-retro-inverso peptide designed to disrupt the FOXO4-p53 interaction inside senescent (zombie) cells, nudging them toward apoptosis. It is the flagship senolytic peptide and is studied in intermittent, pulsed research protocols rather than daily dosing.
Reconstitution
FOXO4-DRI reconstitutes with bacteriostatic water. Draw 2mL down the inside wall of the vial, swirl gently until fully dissolved, and refrigerate. Do not shake.
Quick Reference
| Parameter | Value |
|---|---|
| Peptide Amount | 10mg |
| Reconstitution Solvent | Bacteriostatic water |
| Recommended Volume | 2mL |
| Resulting Concentration | 5mg/mL |
| Typical Research Dose | senolytic pulse |
| Frequency | intermittent (pulsed) |
Reconstitution Guide
Materials Needed
- FOXO4-DRI 10mg lyophilized vial
- Bacteriostatic water (BAC water)
- Insulin syringes (U-100, 29-31 gauge)
- Alcohol swabs
- Sharps container
Step-by-Step Instructions
- Prepare workspace — Clean environment, gather materials, thorough handwashing.
- Clean vial stoppers — Wipe both vials with alcohol swabs. Allow complete drying.
- Draw BAC water — Draw 2mL bacteriostatic water using a sterile syringe.
- Add to peptide — Inject slowly down the inside wall of the vial. Do not spray directly onto the powder.
- Dissolve — Allow a few minutes for complete dissolution. Gentle swirl only — no shaking.
- Verify — Solution should be clear.
- Label and store — Note reconstitution date. Refrigerate immediately.
Dosage Protocol
Concentration Calculations
With 10mg peptide in 2mL BAC water:
- Concentration: 5mg/mL (5000mcg/mL)
- Per unit (U-100 syringe): 50mcg per unit
Run your exact vial and water combination through the peptide reconstitution calculator to rebuild this table for your setup.
Common Dose Calculations
| Desired Dose | Draw Volume | Syringe Units |
|---|---|---|
| 5mg | 1.00mL | 100 units |
| 10mg | 2.00mL | 200 units |
Research Considerations
Mechanism
By displacing p53 from FOXO4 in senescent cells, FOXO4-DRI is proposed to reactivate p53-driven apoptosis selectively in those cells while sparing healthy ones.
Sourcing for Research
Source from verified suppliers that provide third-party testing and certificates of analysis (COA) for each batch. Compare purity documentation, shipping conditions, and batch traceability before purchasing research materials.
Storage Requirements
Lyophilized (Powder)
- Temperature: -20°C or colder
- Duration: 2+ years
- Conditions: Dry, dark, protected from moisture
Reconstituted
- Temperature: 2-8°C (refrigerated)
- Duration: 2-3 weeks
- Conditions: Protected from light, never freeze
Safety Considerations
- FOXO4-DRI is a research compound
- Not approved for any indication in any jurisdiction
- Research use only — not for human consumption
- Use sterile technique; never reuse needles or syringes
Scientific References
- Research literature on FOXO4-DRI and its proposed mechanism of action.
- Peer-reviewed and preclinical sources on the compound class discussed above.
Last updated: 2026-07-20.
Disclaimer: This information is for educational and research purposes only. FOXO4-DRI is a research compound and is not approved for any use. Research-grade peptides are not intended for human consumption. Always follow applicable laws and institutional guidelines.