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🧬 Anti-Aging Last updated 2025-12-23

SS-31 (Elamipretide) 5mg Dosage & Mixing Guide

Also known as: Elamipretide · Forzinity · MTP-131 · Bendavia

Other sizes: 10mg 50mg

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TL;DR

SS-31 (elamipretide) is a mitochondria-targeted peptide studied for its role in cellular energy and mitochondrial function within anti-aging research. It has been evaluated in human clinical trials for specific conditions, with results still emerging and inconclusive. It is a research compound for laboratory investigation and educational reference only, not therapeutic use.

What is SS-31?

SS-31 (Elamipretide, Bendavia, MTP-131) is a mitochondria-targeted tetrapeptide that selectively concentrates in the inner mitochondrial membrane. It targets cardiolipin, a key phospholipid essential for mitochondrial function and energy production.

SS-31 is being studied for various conditions involving mitochondrial dysfunction, including aging, heart failure, and metabolic disorders. It’s one of the most advanced mitochondrial peptides in clinical development.

The 5mg vial provides multiple doses for research protocols.

Quick Reference

ParameterValue
Peptide Amount5mg
Recommended BAC Water1mL
Resulting Concentration5mg/mL
Typical Research Dose0.1-0.5mg/kg
FrequencyOnce daily
Research Duration4-8 weeks

Reconstitution Guide

Step-by-Step Instructions

  1. Clean vial stoppers — Wipe with alcohol swabs
  2. Draw 1mL BAC water — Using sterile syringe
  3. Add to peptide vial — Inject slowly down the wall
  4. Dissolve — Gently swirl, 2-5 minutes
  5. Label and refrigerate — Store immediately

Concentration Calculations

With 5mg in 1mL BAC water:

  • Concentration: 5mg/mL
  • Per unit (U-100): 50mcg per unit

Dosage Protocol

Common Dose Calculations

Desired DoseDraw VolumeSyringe Units
500mcg0.10mL10 units
1mg0.20mL20 units
2.5mg0.50mL50 units

Weight-Based Dosing

For reference (0.25mg/kg example):

  • 60kg: ~15mg
  • 80kg: ~20mg
  • 100kg: ~25mg

Note: Research protocols vary significantly. Dose based on specific study design.

Doses Per Vial

DoseDoses Per Vial
500mcg10 doses
1mg5 doses
2.5mg2 doses

Storage Requirements

FormTemperatureDuration
Lyophilized-20°C2+ years
Reconstituted2-8°C2-3 weeks

Note: SS-31 is sensitive. Use within 2-3 weeks of reconstitution.

Research Considerations

Mechanism of Action

  • Cardiolipin binding — Stabilizes inner mitochondrial membrane
  • Electron transport — Optimizes ATP production
  • ROS reduction — Decreases reactive oxygen species
  • Membrane potential — Maintains mitochondrial function

Research Areas

  • Mitochondrial dysfunction
  • Cardiac health
  • Aging and longevity
  • Neurodegenerative conditions
  • Metabolic disorders

Clinical Development

SS-31 (as Elamipretide) has been in clinical trials for:

  • Barth syndrome
  • Heart failure
  • Age-related macular degeneration
  • Primary mitochondrial myopathy

Sourcing for Research

Source from verified suppliers that provide third-party testing and certificates of analysis (COA) for each batch. Compare purity documentation, shipping conditions, and batch traceability before purchasing research materials.

Research Use Only

SS-31 is a research peptide not intended for human consumption.

Frequently Asked Questions

How do I reconstitute SS-31 5mg?
Add 1mL BAC water of bacteriostatic water. Inject slowly down the side wall. Gently swirl until dissolved — never shake.
What is the typical dosage for SS-31?
Typical research dosage: 0.1-0.5mg/kg. Frequency: Once daily.
How should I store SS-31?
Lyophilized: -20°C (2+ years). Reconstituted: 2-8°C (2-3 weeks). Protect from light.
What concentration does SS-31 5mg make?
With 1mL BAC water BAC water: 5mg/mL. Use our calculator for exact volumes.